We have collected the most exciting new researches in the field of genetics and cellular research in the past week.
Differential expression of coagulation pathway-related proteins in diabetic urine exosomes
Abstract
Background
Coagulation function monitoring is important for the occurrence and development of diabetes. A total of 16 related proteins are involved in coagulation, but how these proteins change in diabetic urine exosomes is unclear. To explore the expression changes of coagulation-related proteins in urine exosomes and their possible roles in the pathogenesis of diabetes, we performed proteomic analysis and finally applied them to the noninvasive monitoring of diabetes.
Methods
Subject urine samples were collected. LC-MS/MS was used to collect the information on coagulation-related proteins in urine exosomes. ELISA, mass spectrometry and western blotting were used to further verify the differential protein expression in urine exosomes. Correlations with clinical indicators were explored, and receiver operating characteristic (ROC) curves were drawn to evaluate the value of differential proteins in diabetes monitoring.
Results
Analyzing urine exosome proteomics data, eight coagulation-related proteins were found in this study. Among them, F2 was elevated in urine exosomes of diabetic patients compared with healthy controls. The results of ELISA, mass spectrometry and western blotting further verified the changes in F2. Correlation analysis showed that the expression of urine exosome F2 was correlated with clinical lipid metabolism indexes, and the concentration of F2 was strongly positively correlated with blood TG levels (P < 0.05). ROC curve analysis showed that F2 protein in urine exosomes had a good monitoring value for diabetes.
Conclusion
Coagulation-related proteins were expressed in urine exosomes. Among them, F2 was increased in diabetic urine exosomes and may be a potential biomarker for monitoring diabetic changes.
How do tumor exosomes communicate cancer progression?
Abstract
Exosomes are extracellular vesicles ranging between 30 and 150 nm in diameter that are released from different kinds of cells, including tumor cells. They have the potential to induce apoptosis, modulate the immune system, and work as biomarkers for diagnosis. As a key component of cell-to-cell communication, exosomes can control the tumor microenvironment and contribute immensely to the development, progression, and metastasis processes of numerous cancers.
The biological function of tumor-derived extracellular vesicles on metabolism
In the world of skincare, we constantly are reminded of the word "exosomes." Consequently, we assume that exosomes are great for our skin, otherwise, why else would they be mentioned? Thankfully, this assumption is correct, but have you ever taken a moment to understand what the benefits of exosomes are for your skin's health, or even what an exosome is? If not, there is no need to worry, we've got you covered. At The Skin Room, we frequently discuss exosomes, and that is not solely because our favorite product we carry happens to be filled with them (wink). So, let's get talking about what exactly exosomes can do for your skin.