Exosomes Digest (2/4 January 2025)
- Lisa
- 20. Jan.
- 7 Min. Lesezeit
We have collected the most exciting new researches in the field of genetics and cellular research in the past week.
Exosomes and microRNAs as mediators of the exercise
MicroRNAs (miRNAs), also known as microribonucleic acids, are small molecules found in specific tissues that are essential for maintaining proper control of genes and cellular processes. Environmental factors, such as physical exercise, can modulate miRNA expression and induce targeted changes in gene transcription. This article presents an overview of the present knowledge on the principal miRNAs influenced by physical activity in different tissues and bodily fluids. Numerous research projects have emphasized the significant impact of miRNAs on controlling biological changes brought about by physical activity. These molecules play main roles in important processes such as the growth of skeletal muscle and heart muscle cells, the creation of mitochondria, the development of the vascular system, and the regulation of metabolism. Studies have shown that physical exertion utilizes the contributions of miR-1, miR-133, miR-206, miR-208, and miR-486 to revitalize and restore skeletal muscle tissue. Moreover, detecting alterations in miRNA levels and connecting them to the specific outcomes of various exercise routines and intensities can act as indicators for physical adaptation and the reaction to physical activity in long-term diseases. Numerous studies have confirmed that extracellular vesicles (EVs) which composed of different members such as exosomes have the ability to reduce inflammation through the activation of the nuclear factor kappa B (NF-κB pathway. Furthermore, physical activity greatly affects the levels of specific miRNAs present in exosomes derived from skeletal muscle. Therefore, exosomal miRNAs target some pathways that are related to growth and development, such asWnt/β-catenin, PI3K/AKT, and insulin-like growth factor 1 (IGF1). Exercise-induced exosomes have also been identified as important mediators in promoting beneficial effects throughout the body. The aim of this review is to summarize the effect of exercise on the function of miRNAs and exosomes.
Exosomes: Historical Evolution and Emerging Roles in Dermatology
Background
Exosomes are a nanoscale extracellular vesicles derived from different cell types that have been investigated for various clinical applications, including functioning as biomarkers and use as direct therapeutics. Given the role of exosomes in multiple pathophysiologic pathways and potential practical applications, they have garnered significant interest in the scientific community but much is still unknown about their development and use.
Aims
This literature review covers the background, mechanisms of action, use as biomarkers, methods of application, and direct therapeutic applications of exosomes.
Methods
A literature review on the background and uses of exosomes was conducted. Key articles describing the pathophysiologic pathways and applications of exosomes were summarized and described.
Results
Exosomes impact several cellular pathways which allow them to function as biomarkers for malignancy and inflammatory dermatoses and may make them useful therapeutics for skin rejuvenation, hair loss, and wound repair. Limitations of exosomes include an incomplete understanding of their functions and impacts and a lack of standardization in their production and application.
Conclusions
Exosomes are a unique and novel cellular medium that offer promise as a diagnostic tool and therapy. While there are limitations to the uses of exosomes as well as our current understanding of them, further investigation may yield additional applications and a larger role in medicine for exosomes.
The roles and therapeutic potential of exosomal non-coding RNAs in microglia-mediated intercellular communication
Exosomes, which are small extracellular vesicles (sEVs), serve as versatile regulators of intercellular communication in the progression of various diseases, including neurological disorders. Among the diverse array of cargo they carry, non-coding RNAs (ncRNAs) play key regulatory roles in various pathophysiological processes. Exosomal ncRNAs derived from distinct cells modulate their reciprocal crosstalk locally or remotely, thereby mediating neurological diseases. Nevertheless, the emerging role of exosomal ncRNAs in microglia-mediated phenotypes remains largely unexplored. This review aims to summarise the biological functions of exosomal ncRNAs and the molecular mechanisms that underlie their impact on microglia-mediated intercellular communication, modulating neuroinflammation and synaptic functions within the landscape of neurological disorders. Furthermore, this review comprehensively described the potential applications of exosomal ncRNAs as diagnostic and prognostic biomarkers, as well as innovative therapeutic targets for the treatment of neurological diseases.
Role of Exosomes in the Progression, Diagnosis, and Therapy Targeting of Malignant Brain Gliomas
Glioblastoma is the most common primary tumor of the central nervous system, characterized by an infiltrative growth pattern, which results in the most unfavorable prognosis. The average survival time of patients after diagnosis of this tumor is typically several months, with complete recovery from glioma being very rare. In recent years, significant involvement of exosomes in the development of cancer, including malignant brain tumors, has been discovered. Exosomes are extracellular vesicles that carry signaling molecules and participate in communication between cells. They influence cell survival, proliferation, migration, and increased neoangiogenesis, all of which significantly contribute to tumor recurrence. Molecules carried by exosomes are considered potential diagnostic markers, enabling early diagnosis of cancer and prompt implementation of appropriate treatment. Of particular diagnostic importance are microRNA molecules, which promote increased cell proliferation and inhibition of apoptosis. Equally important exosomal transmitters include proteins such as PSMD2 and EGFR, which enhance tumor invasiveness and resistance to chemotherapeutic agents. Recent studies suggest the possibility of using exosomes as carriers for new anticancer drugs, potentially improving the therapeutic treatment of cancers resistant to standard treatment methods. This review aimed to provide a comprehensive analysis of recent research on glioblastoma, the role of exosomes in its progression, the potential of exosomes as diagnostic biomarkers, and their use as therapeutic targets for patients who have not responded to conventional treatments.
Exosomes: Key Messengers Mediating the Interaction Between Tumor Cells and CD8+ T Cells in the Tumor Microenvironment
In recent years, with an increasingly profound comprehension of the tumor microenvironment, it has been discovered that the constituent cells within the immune microenvironment, such as macrophages, CD4+T cells, and CD8+T cells, interact with tumor cells in manners conducive to tumorigenesis and progression. Exosomes play a pivotal role as essential mediators for intercellular material exchange and signal transmission in this context. Tumor cell-derived exosomes carrying cargo such as PD-L1 and ncRNAs engage with CD8+T cells to induce cytotoxic responses and facilitate immune evasion, thereby promoting tumor advancement. When combined with current immune checkpoint inhibitors like anti-PD-L1/PD-1 therapy, enhancing CD8+T cell function through exosomal pathways while monitoring and augmenting therapeutic effects can significantly improve efficacy. This review delineates the crucial role of exosomes derived from both tumor cells and CD8+T cells within the tumor microenvironment along with their impact mechanisms on both tumor cells and CD8+T cells. Furthermore, it summarizes the potential for clinical treatment in this realm when integrated with existing immunotherapy methods—particularly exploring the feasibility of clinical translation alongside engineering materials science techniques.
Exosome Proteomics: A Key to Understanding Disease Mechanisms and Enhancing Therapeutic Interventions
Exosomes, small extracellular vesicles ranging from 30–150 nm in diameter, have become focal points in modern biomedical research. These nanoscale entities, secreted by most cell types, are involved in cellular communication and carry a variety of biomolecules, such as proteins, lipids, RNA, and even DNA. Their biological relevance has been well-documented, with increasing evidence pointing to their potential in diagnostics, disease monitoring, and therapeutic applications. Among the many bioactive molecules exosomes contain, their proteomic cargo is particularly valuable. This article provides an in-depth exploration of exosome proteomics, its applications in understanding diseases, and the evolving challenges and opportunities in utilizing exosome-derived proteins for therapeutic purposes.
Application of exosomes for the regeneration of skin wounds: Principles, recent applications and limitations
In the medical field, wound healing poses significant challenges due to its complexity and time-consuming nature. Cell-free wound repair, notably the utilization of exosomes (EXOs), has made significant progress in recent years. Urine, saliva, umbilical cord, blood, mesenchymal stem cells and breast milk cells can be used to extract and purify EXOs, which are Nano-sized lipid bilayer vesicles. Besides their relatively little toxicity, non-specific immunogenicity and excellent biocompatibility, EXOs also contain bioactive molecules such as proteins, lipids, microRNAs (miRNAs), and messenger RNAs (mRNAs). Their bioactive compounds have anti-inflammatory properties and can speed up wound healing. Various medicinal agents can also be contained within the EXOs. This review briefly provides new information on the different aspects of EXOs and evaluate the application of EXOs as a promising therapy in the regeneration of skin wounds in recent pre-clinical and clinical studies.
Exosomes: the insulin of our era?
Initially believed to be a mechanism for cells to “take out the trash,” the latest research in the exosome field indicates that minute, extracellular vesicles released by all living cells have the power to unlock a host of transformative possibilities as markers of disease and successful therapeutics.
The University of Miami Miller School of Medicine community came together for a specialized topic research retreat, with four exosome researchers presenting “Biological and Translational Application of Exosomes,” followed by a panel discussion.
The retreat shared updated information about exosomes with Miller School personnel across all disciplines. Attendees gained perspective on how the untapped potential of exosomes can improve both research and diagnostics.
Researchers launch startup to revolutionize targeted drug delivery using milk exosomes
Two Nebraska researchers have launched a startup company aimed at bringing to market an innovative method for delivering therapeutics, gene editing tools, plasmids and more to targeted locations in the human body.
Minovacca, recently incorporated by Janos Zempleni and Jiantao Guo, will commercialize the use of universal milk exosomes - natural nanoparticles contained in milk - to transport cargo to human cells. Drawing on bioorthogonal chemistry techniques, the researchers achieve target-specific delivery by chemically and genetically engineering the exosomes. NUtech Ventures, the university's nonprofit commercialization affiliate, has filed a patent for the technology.
Effects and mechanisms of exosomes in microenvironment angiogenesis in breast cancer: An updated review
Exosomes (EXOs) play an important role in the progression of breast cancer. EXOs, with a diameter of approximately 100 nm, have a simple structure but diverse functions, and can affect the development of breast cancer through signal transduction and molecular transfer, etc. Angiogenesis provides nutrients for the growth and metastasis of breast cancer and is a crucial part of tumor progression. The mechanism of tumor angiogenesis is complex. The VEGF/VEGFR pathway promotes angiogenesis by regulating the activities of ECs. Hypoxia, a common feature in the tumor microenvironment, as a key regulator, can affect angiogenesis in multiple aspects such as the transfer of miRNAs in EXOs, protein transport, extracellular matrix regulation, and metabolic adaptation. The Notch pathway has a bidirectional regulatory role in breast cancer angiogenesis, and different molecules can promote or inhibit angiogenesis. EXOs secreted by breast cancer cells are rich in angiogenic factors. Components such as proteins and nucleic acids in EXOs can affect the functions and behaviors of vascular ECs, thereby influencing breast cancer angiogenesis. Research on the mechanisms of EXOs in breast cancer angiogenesis is of great significance for tumor treatment. EXOs are expected to become biomarkers for breast cancer diagnosis/prognosis. This research provides potential targets for in-depth understanding of the biological characteristics of breast cancer and the development of new treatment strategies.